• Nogami Ryōtarō (野上 良太郎, Nogami Ryōtarō?): Satō Takeru (佐藤 健, Satō Takeru?)
• Hana (ハナ, Hana?): Shiratori Yuriko (白鳥 百合子, Shiratori Yuriko?)
• Kohana (コハナ, Kohana?): Matsumoto Tamaki (松元 環季, Matsumoto Tamaki?)
• Naomi (ナオミ, Naomi?): Akiyama Rina (秋山 莉奈, Akiyama Rina?)
• Owner/Người kể chuyện đầu phim/Trạm trưởng (オーナー／オープニングナレーション／駅長, Ōnā/Ōpuningu Narēshon/Ekichō?): Ishimaru Kenjirō (石丸 謙二郎, Ishimaru Kenjirō?)
• Nogami Airi (野上 愛理, Nogami Airi?): Matsumoto Wakana (松本 若菜, Matsumoto Wakana?)
• Ozaki Seigi (尾崎 正義, Ozaki Seigi?): Nagata Akira (永田 彬, Nagata Akira?) of Run&Gun|RUN&GUN
• Miura Issē (三浦 イッセー, Miura Issē?): Ueno Ryō (上野 亮, Ueno Ryō?)
• Sakurai Yūto (桜井 侑斗, Sakurai Yūto?): Nakamura Yūichi (中村 優一, Nakamura Yūichi?)
• Người trong quá khứ (過去の男, Kako no Otoko?): Okano Tomonobu (岡野 友信, Okano Tomonobu?)
• Kai (カイ, Kai?): Ishiguro Hideo (石黒 英雄, Ishiguro Hideo?)

Diễn viên lồng tiếng

Nhờ sự thể hiện các Tarōs của Seki Toshihiko, Yusa Kōji, Terasoma Masaki, và Suzumura Kenichi, Kamen Rider Den-O đã giành Giải thưởng Synergy ở Seiyu Awards lần thứ hai.[4]

• Momotaros (モモタロス, Momotarosu?): Seki Toshihiko (関 俊彦, Seki Toshihiko?)
• Urataros (ウラタロス, Uratarosu?): Yusa Kōji (遊佐 浩二, Yusa Kōji?)
• Kintaros (キンタロス, Kintarosu?): Terasoma Masaki (てらそま まさき, Terasoma Masaki?)
• Ryutaros (リュウタロス, Ryūtarosu?): Suzumura Ken'ichi (鈴村 健一, Suzumura Ken'ichi?)
• Deneb (デネブ, Denebu?): Ōtsuka Hōchū (大塚 芳忠, Ōtsuka Hōchū?)
• Sieg (ジーク, Jīku?, 23-24, 49): Miki Shin'ichirō (三木 眞一郎, Miki Shin'ichirō?)

Diễn viên đóng thế

• Kamen Rider Den-O/Momotaros: Takaiwa Seiji (高岩 成二, Takaiwa Seiji?)
• Urataros: Eitoku (永徳, Eitoku?)
• Kintaros: Okamoto Jirō (岡元 次郎, Okamoto Jirō?)
• Ryutaros: Ogura Toshihiro (おぐら としひろ, Ogura Toshihiro?)
• Kamen Rider Zeronos: Itō Makoto (伊藤 慎, Itō Makoto?)
• Deneb: Oshikawa Yoshifumi (押川 善文, Oshikawa Yoshifumi?)
• Sieg: Nagase Naoki (永瀬 尚希, Nagase Naoki?)

Diễn viên khách mời

• Yamagoshi Yū (山越 佑, Yamagoshi Yū?) (3-4): Namioka Kazuki (波岡 一喜, Namioka Kazuki?)
• Saitō Yumi (斉藤 優美, Saitō Yumi?) (7-8): Kurihara Hitomi (栗原 瞳, Kurihara Hitomi?)
• Honjō Masaru (本条 勝, Honjō Masaru?) (9-10): Uchino Kenta (内野 謙太, Uchino Kenta?)
• Toyama Shūji (戸山 秀二, Toyama Shūji?) (13-14): Ueda Shun (うえだ 峻, Ueda Shun?)
• Hakamada Shūjirō (袴田 秀次郎, Hakamada Shūjirō?) (15-16): Ōshiba Hayato (大柴 隼人, Ōshiba Hayato?)
• Sawada Yuka (沢田 由香, Sawada Yuka?) (17-18, 33): Mitsushima Hikari (満島 ひかり, Mitsushima Hikari?)
• Gaoh (牙王, Gaō?) (25-28): Watanabe Hiroyuki (渡辺 裕之, Watanabe Hiroyuki?)
• Aoki Masashi (青木 雅史, Aoki Masashi?) (25-26): Yamamoto Kōhei (山本 康平, Yamamoto Kōhei?)
• Machida (町田, Machida?) (29): Sugawara Takuma (菅原 卓磨, Sugawara Takuma?)
• Terasaki Tōru (寺崎 トオル, Terasaki Tōru?) (30): Hayashi Tsuyoshi (林 剛史, Hayashi Tsuyoshi?)
• Yamaguchi (山口, Yamaguchi?) (35-36): Furuya Yōichi (古屋 暢一, Furuya Yōichi?)

Diễn viên lồng tiếng khách mời

• Bat Imagin (バットイマジン, Batto Imajin?, 1-2): Yanada Kiyoyuki (梁田 清之, Yanada Kiyoyuki?)
• Crust Imagin (クラストイマジン, Kurasuto Imajin?, 5-6): Tsukui Kyōsei (津久井 教生, Tsukui Kyōsei?)
• Crow Imagin (クロウイマジン, Kurou Imajin?, 7-8): Nishi Rintarō (西 凛太朗, Nishi Rintarō?)
• Rhino Imagin (ライノイマジン, Raino Imajin?, 9-10): Koyama Tsuyoshi (小山 剛志, Koyama Tsuyoshi?)
• Owl Imagin (オウルイマジン, Ouru Imajin?, 13-14), Albinoleo Imagin (アルビノレオイマジン, Arubinoreo Imajin?, 43-46): Kuroda Takaya (黒田 崇矢, Kuroda Takaya?)
• Wolf Imagin (ウルフイマジン, Urufu Imajin?, 17-18): Hiyama Nobuyuki (檜山 修之, Hiyama Nobuyuki?)
• Jelly Imagin (ジェリーイマジン, Jerī Imajin?, 19-20): Nakao Ryūsei (中尾 隆聖, Nakao Ryūsei?)
• Tortoice Imagin (トータスイマジン, Tōtasu Imajin?, 21-22), Panda Rabbit Imagin (パンダラビットイマジン, Panda Rabitto Imajin?, 39): Ochiai Kōji (落合 弘治, Ochiai Kōji?)
• Molech Imagin (モレクイマジン, Moreku Imajin?, 27): Tokuyama Hidenori (徳山 秀典, Tokuyama Hidenori?)
• Kraken Imagin (クラーケンイマジン, Kurāken Imajin?, 33-34): Inada Tetsu (稲田 徹, Inada Tetsu?)
• Leo Imagin (レオイマジン, Reo Imajin?, 37-38): Yamaji Kazuhiro (山路 和弘, Yamaji Kazuhiro?)
• Death Imagin (デスイマジン, Desu Imajin?, 48-49): Yanaka Hiroshi (家中 宏, Yanaka Hiroshi?)